pB-TAG-NICD | Addgene 130934 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pB-TAG-NICD

catalog :

130934

citations: 3

Reference
Liu Z, Shan S, Yuan Z, Wu F, Zheng M, Wang Y, et al. Mitophagy bridges DNA sensing with metabolic adaption to expand lung cancer stem-like cells. EMBO Rep. 2022;:e54006 pubmed publisher
Kumar V, Vashishta M, Kong L, Lu J, Wu X, Dwarakanath B, et al. Carbon Ion Irradiation Downregulates Notch Signaling in Glioma Cell Lines, Impacting Cell Migration and Spheroid Formation. Cells. 2022;11: pubmed publisher
Chu L, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, et al. An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells. Cell Rep. 2019;28:2247-2255.e5 pubmed publisher

product information

Catalog Number :

130934

Product Name :

pB-TAG-NICD

article :

doi	10.1016/j.celrep.2019.07.090
id	28203954
pubmed_id	31461642

bacterial resistance :

Ampicillin

cloning :

backbone

pB-TAG-ERP2

backbone_mutation

backbone_origin

addgene 80479

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

growth temp :

Note: This plasmid is prone to recombination. We recommend testing 2-4 colonies via restriction digest to ensure the full plasmid is intact.

origin :

pi :

alt_names

Notch intracellular domain

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	TetO
sequencing_primer_3	GTCGTCCTTGAAGAAGATGGT
sequencing_primer_5	acgtctagaacgtctccctatc
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AOS5, AOVD1, TAN1, hN1
gene	NOTCH1
id	4851

genbank_ids

mutation

name

NICD

shRNA_sequence

size

2412

species

9606

Homo sapiens