This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKM208
catalog :
13077
citations: 7
Reference
Thomas S, Madaan T, Kamble N, Siddiqui N, Pauletti G, Kotagiri N. Engineered Bacteria Enhance Immunotherapy and Targeted Therapy through Stromal Remodeling of Tumors. Adv Healthc Mater. 2021;:e2101487 pubmed publisher
Ibraheim R, Tai P, Mir A, Javeed N, Wang J, Rodríguez T, et al. Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo. Nat Commun. 2021;12:6267 pubmed publisher
Doore S, Subramanian S, Tefft N, Morona R, Teravest M, Parent K. Large metabolic rewiring from small genomic changes between strains of Shigella flexneri. J Bacteriol. 2021;: pubmed publisher
Campellone K, Coulter A. Lambda Red-Mediated Recombination in Shiga Toxin-Producing Escherichia coli. Methods Mol Biol. 2021;2291:145-162 pubmed publisher
Aeschbach L, Dion V. Minimizing carry-over PCR contamination in expanded CAG/CTG repeat instability applications. Sci Rep. 2017;7:18026 pubmed publisher
Hartwich H, Nothwang H. An easy and versatile 2-step protocol for targeted modification and subcloning of DNA from bacterial artificial chromosomes using non-commercial plasmids. BMC Res Notes. 2012;5:156 pubmed publisher
Murphy K, Campellone K. Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. BMC Mol Biol. 2003;4:11 pubmed
product information
Catalog Number :
13077
Product Name :
pKM208
article :
doi10.1186/1471-2199-4-11
id997
pubmed_id14672541
bacterial resistance :
Ampicillin
cloning :
backbonepKM201
backbone_mutation
backbone_originKenan Murphy
backbone_size7200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
lacI gene obtained from plasmid pTP550
growth temp :
Plasmid contains temperature sensitive origin of replication and must be grown at 30C
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoter
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0345, ECK0342
genelacI
id945007
genbank_ids
J01636
mutation
namelacI repressor
shRNA_sequence
size1400
species
E. coli
tags
plasmid copy :
Anthony Poteete UMass Medical School
resistance markers :
352
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA