pLVH-EF1a-GFP-P2A-MYC | Addgene 130695 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVH-EF1a-GFP-P2A-MYC

catalog :

130695

citations: 3

Reference
Hossain M, Cevallos R, Zhang R, Hu K. Attenuating iPSC reprogramming stress with dominant-negative BET peptides. iScience. 2023;26:105889 pubmed publisher
Cevallos R, Edwards Y, Parant J, Yoder B, Hu K. Human transcription factors responsive to initial reprogramming predominantly undergo legitimate reprogramming during fibroblast conversion to iPSCs. Sci Rep. 2020;10:19710 pubmed publisher
Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher

product information

Catalog Number :

130695

Product Name :

pLVH-EF1a-GFP-P2A-MYC

article :

doi	10.1038/ncomms10869
id	17970
pubmed_id	26947130

bacterial resistance :

Ampicillin

cloning :

backbone

pLVH-EF1a-GFP-P2A

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

We extensively modified the plasmid of pLVX-AcGFP-C1 (Clontech, 632155) by replacing CMV promoter with EF1a promoter, adding P2A to mediate co-expression of the gene of interest, and removal of PGK promoter and puromycin resistant gene to make a lentiviral backbone vector of pLVH-EF1a-P2A.

growth strain :

Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

origin :

pi :

alt_names

cloning

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

name

human MYC

shRNA_sequence

size

1320

species

9606

Homo sapiens