pLVH-EF1a-GFP-P2A-OCT4 | Addgene 130692 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVH-EF1a-GFP-P2A-OCT4

catalog :

130692

citations: 4

Reference
Hossain M, Cevallos R, Zhang R, Hu K. Attenuating iPSC reprogramming stress with dominant-negative BET peptides. iScience. 2023;26:105889 pubmed publisher
Cevallos R, Edwards Y, Parant J, Yoder B, Hu K. Human transcription factors responsive to initial reprogramming predominantly undergo legitimate reprogramming during fibroblast conversion to iPSCs. Sci Rep. 2020;10:19710 pubmed publisher
Lee H, Lee H, Lee B, Gerovska D, Park S, Zaehres H, et al. Sequentially induced motor neurons from human fibroblasts facilitate locomotor recovery in a rodent spinal cord injury model. elife. 2020;9: pubmed publisher
Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher

product information

Catalog Number :

130692

Product Name :

pLVH-EF1a-GFP-P2A-OCT4

article :

doi	10.1038/ncomms10869
id	17970
pubmed_id	26947130

bacterial resistance :

Ampicillin

cloning :

backbone

pLVH-EF1a-GFP-P2A

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

We extensively modified the plasmid of pLVX-AcGFP-C1 (Clontech, 632155) by replacing CMV promoter with EF1a promoter, adding P2A to mediate co-expression of the gene of interest, and removal of PGK promoter and puromycin resistant gene to make a lentiviral backbone vector of pLVH-EF1a-P2A.

growth strain :

Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

origin :

pi :

alt_names

POU5F1

cloning

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

mutation

name

human OCT4

shRNA_sequence

size

1080

species

9606

Homo sapiens