This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPICZ:hTRAAK(W262S)
catalog :
130670
citations: 1
Reference
Lolicato M, Riegelhaupt P, Arrigoni C, Clark K, Minor D. Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels. Neuron. 2014;84:1198-212 pubmed publisher
product information
Catalog Number :
130670
Product Name :
pPICZ:hTRAAK(W262S)
article :
doi10.1016/j.neuron.2014.11.017
id28203918
pubmed_id25500157
bacterial resistance :
Bleocin (Zeocin)
cloning :
backbonepPICZ
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoter
sequencing_primer_3AOX3
sequencing_primer_5AOX5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFHEIG, K2p4.1, TRAAK, TRAAK1
geneKCNK4
id50801
genbank_ids
mutationN104Q, N108Q, W262S
nameKCNK4
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagSNS- 3C_cleavage_site-TAAA-GFP
resistance markers :
3967
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA