This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
28 pCS plcg 1
catalog :
13059
citations: 1
| Reference |
|---|
Lawson N, Mugford J, Diamond B, Weinstein B. phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Genes Dev. 2003;17:1346-51 pubmed
|
product information
Catalog Number :
13059
Product Name :
28 pCS plcg 1
article :
| doi | 10.1101/gad.1072203 |
| id | 1009 |
| pubmed_id | 12782653 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pCS2+ |
| backbone_mutation | |
| backbone_origin | |
| backbone_size | 4100 |
| promoter | |
| sequencing_primer_3 | |
| sequencing_primer_5 | |
| vector_types |
growth notes :
The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. Additional plcg1 cDNA sequence was obtained or confirmed by cycle sequencing of reverse transcriptase PCR (RT
origin :
37
pi :
|
resistance markers :
| 275 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments