27 pCR plcg 1 CDS | Addgene 13058 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

27 pCR plcg 1 CDS

catalog :

13058

citations: 1

Reference
Lawson N, Mugford J, Diamond B, Weinstein B. phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Genes Dev. 2003;17:1346-51 pubmed

product information

Catalog Number :

13058

Product Name :

27 pCR plcg 1 CDS

article :

doi	10.1101/gad.1072203
id	1009
pubmed_id	12782653

bacterial resistance :

Ampicillin

cloning :

backbone	pCR2.1
backbone_mutation
backbone_origin	Invitrogen
backbone_size	3900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. For riboprobe, digest with MluI and use SP6 polymerase.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7
sequencing_primer_5	M13 rev
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	fj46f04, wu:fj46f04, wu:fj57a05
gene	plcg1
id	373867

genbank_ids

mutation

name

plcg1

shRNA_sequence

size

species

7955

Danio rerio