This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-PTEN
catalog :
13039
citations: 16
Reference
Dimou A, Nasarre C, Peterson Y, Pagano R, Gooz M, Nasarre P, et al. Neuropilin-2b facilitates resistance to tyrosine kinase inhibitors in non-small cell lung cancer. J Thorac Cardiovasc Surg. 2020;: pubmed publisher
Ghezzi C, Wong A, Chen B, Ribalet B, Damoiseaux R, Clark P. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Nat Commun. 2019;10:5444 pubmed publisher
Luo S, Lei K, Xiang D, Ye K. NQO1 Is Regulated by PTEN in Glioblastoma, Mediating Cell Proliferation and Oxidative Stress. Oxid Med Cell Longev. 2018;2018:9146528 pubmed publisher
Wu Y, Lin C, Lin Y, Su W, Chiu W. Autophagy regulates vinorelbine sensitivity due to continued Keap1-mediated ROS generation in lung adenocarcinoma cells. Cell Death Discov. 2018;4:33 pubmed publisher
Yu C, Zhang B, Li Y, Yu X. SIX1 reduces the expression of PTEN via activating PI3K/AKT signal to promote cell proliferation and tumorigenesis in osteosarcoma. Biomed Pharmacother. 2018;105:10-17 pubmed publisher
El Sharkawi F, Ewais S, Fahmy R, Rashed L. PTEN and TRAIL genes loaded zein nanoparticles as potential therapy for hepatocellular carcinoma. J Drug Target. 2017;25:513-522 pubmed publisher
Gemmill R, Nasarre P, Nair Menon J, Cappuzzo F, Landi L, D Incecco A, et al. The neuropilin 2 isoform NRP2b uniquely supports TGF?-mediated progression in lung cancer. Sci Signal. 2017;10: pubmed publisher
Moore B, Stepanchick A, Tewson P, Hartle C, Zhang J, Quinn A, et al. Cilia have high cAMP levels that are inhibited by Sonic Hedgehog-regulated calcium dynamics. Proc Natl Acad Sci U S A. 2016;113:13069-13074 pubmed
van Ree J, Nam H, Jeganathan K, Kanakkanthara A, van Deursen J. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol. 2016;18:814-21 pubmed publisher
Masters T, Sheetz M, Gauthier N. F-actin waves, actin cortex disassembly and focal exocytosis driven by actin-phosphoinositide positive feedback. Cytoskeleton (Hoboken). 2016;73:180-96 pubmed publisher
Conde Perez A, Gros G, Longvert C, Pedersen M, Petit V, Aktary Z, et al. A caveolin-dependent and PI3K/AKT-independent role of PTEN in β-catenin transcriptional activity. Nat Commun. 2015;6:8093 pubmed publisher
Zhang Y, Ueno Y, Liu X, Buller B, Wang X, Chopp M, et al. The MicroRNA-17-92 cluster enhances axonal outgrowth in embryonic cortical neurons. J Neurosci. 2013;33:6885-94 pubmed publisher
Mathews M, Shih E, Zamora G, Sun C, Cho S, Kwon Y, et al. Glioma cell growth inhibition following photochemical internalization enhanced non-viral PTEN gene transfection. Lasers Surg Med. 2012;44:746-54 pubmed publisher
Li P, Peng H. Regulation of axonal growth and neuromuscular junction formation by neuronal phosphatase and tensin homologue signaling. Mol Biol Cell. 2012;23:4109-17 pubmed publisher
Howitt J, Lackovic J, Low L, Naguib A, Macintyre A, Goh C, et al. Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia. J Cell Biol. 2012;196:29-36 pubmed publisher
Liu F, Wagner S, Campbell R, Nickerson J, Schiffer C, Ross A. PTEN enters the nucleus by diffusion. J Cell Biochem. 2005;96:221-34 pubmed
product information
Catalog Number :
13039
Product Name :
GFP-PTEN
article :
doi10.1002/jcb.20525
id984
pubmed_id16088943
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
GFP-PTEN was cloned into pcDNA3 as two fragments. The enzyme/insert order is: HindIII - GFP - BamHI - PTEN - EcoRI.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1
genePTEN
id5728
genbank_ids
NM_000314
mutation
namePTEN
shRNA_sequence
size1900
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
425
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA