pCFD3-Act5c | Addgene 130278 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCFD3-Act5c

catalog :

130278

citations: 2

Reference
Bosch J, Knight S, Kanca O, ZIRIN J, Yang Zhou D, Hu Y, et al. Use of the CRISPR-Cas9 System in Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes. Curr Protoc Mol Biol. 2020;130:e112 pubmed publisher
Bosch J, Colbeth R, ZIRIN J, Perrimon N. Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids. Genetics. 2019;: pubmed publisher

product information

Catalog Number :

130278

Product Name :

pCFD3-Act5c

article :

doi	10.1534/genetics.119.302819
id	28203904
pubmed_id	31685521

bacterial resistance :

Ampicillin

cloning :

backbone

pCFD3

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

CRISPR

growth notes :

There is a mixed nucleotide population for a single bp in vermillion. If the plasmid contains a C, there is a S322P SNP in vermillion. Depositor confirms this does not affect plasmid function.

growth strain :

Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

origin :

pi :

alt_names

GP07595

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	Unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG4027, A, A4V404_DROME, ACT, ACT1_DROME, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, ArpA, BAP47, Bap47, CG4027, Dmel CG4027, F-ACTIN, F-Actin, F-actin, G-actin, M32055, T11, act, act 5C, act5C, actin, actin5C, actomyosin, anon-EST:fe2D2, beta-Actin, beta-actin, beta-actin/Bap47, chrX:5748184. 5748304, cyt5C, f-actin, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486
gene	Act5C
id	31521

genbank_ids

mutation

name

pCFD3-Act5c

shRNA_sequence

size

species

7227

Drosophila melanogaster