This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
G30, Cdc20-mCherry-AID tagging
catalog :
130270
citations: 1
Reference
Özsezen S, Papagiannakis A, Chen H, Niebel B, Milias Argeitis A, Heinemann M. Inference of the High-Level Interaction Topology between the Metabolic and Cell-Cycle Oscillators from Single-Cell Dynamics. Cell Syst. 2019;9:354-365.e6 pubmed publisher
product information
Catalog Number :
130270
Product Name :
G30, Cdc20-mCherry-AID tagging
article :
doi10.1016/j.cels.2019.09.003
id28203900
pubmed_id31606371
bacterial resistance :
Ampicillin
cloning :
backboneHO-poly-KanMX4-HO
backbone_mutationThe HO homologous arms are replaced by Cdc20 homologous arms for genomic integration.
backbone_originhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/
backbone_size2531
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
This plasmid can be used to dynamically and conditionally deplete Cdc20 in yeast. The work is currently under review.
growth strain :
Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination
origin :
37
pi :
alt_names
YGL116W
PAC5
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterNative promoter
sequencing_primer_3CCATAATGGCGCAGAG
sequencing_primer_5AGCACTAAGCTGGTGTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGL116W, PAC5
geneCDC20
id852762
genbank_ids
mutation
nameCdc20/mCherry-AID(71-114)-tCYC1-NatMX
shRNA_sequence
size2328
species
4932
Saccharomyces cerevisiae
100
Synthetic
tags
locationC terminal on insert
tagmCherry
locationC terminal on insert
tagAID(71-114)
plasmid copy :
The AID(71-114) sequence was retrieved from: Morawska, M., and Ulrich, H.D. (2013). An expanded tool kit for the auxin-inducible degron system in budding yeast. Yeast 30, 341 351. mCherry was retrieved from the pBS35 plasmid.
resistance markers :
3411
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA