This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EGFP-Cdc42-Q61L
catalog :
12986
citations: 6
Reference
Liu L, Sui R, Li L, Zhang L, Zeng D, Ni X, et al. Light Activates Cdc42-Mediated Needle-Shaped Filopodia Formation via the Integration of Small GTPases. Cell Mol Bioeng. 2022;15:599-609 pubmed publisher
Rossatti P, Ziegler L, Schregle R, Betzler V, Ecker M, Rossy J. Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane. Cells. 2019;8: pubmed publisher
Liu L, Jiang H, Zhao W, Meng Y, Li J, Huang T, et al. Cdc42-mediated supracellular cytoskeleton induced cancer cell migration under low shear stress. Biochem Biophys Res Commun. 2019;519:134-140 pubmed publisher
Rovira Clavé X, Angulo Ibáñez M, Reina M, Espel E. The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation. Cell Signal. 2014;26:1489-99 pubmed publisher
Dehapiot B, Carriere V, Carroll J, Halet G. Polarized Cdc42 activation promotes polar body protrusion and asymmetric division in mouse oocytes. Dev Biol. 2013;377:202-12 pubmed publisher
Subauste M, Von Herrath M, Benard V, Chamberlain C, Chuang T, Chu K, et al. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. J Biol Chem. 2000;275:9725-33 pubmed
product information
Catalog Number :
12986
Product Name :
pcDNA3-EGFP-Cdc42-Q61L
article :
doi10.1074/jbc.275.13.9725
id964
pubmed_id10734125
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-EGFP
backbone_mutation
backbone_originInvitrogen
backbone_size6100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Cdc42
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7 for EGFP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDC42Hs, G25K, TKS
geneCDC42
id998
genbank_ids
M57298
mutationQ61L Constitutively active
nameCdc42 constitutively active
shRNA_sequence
size576
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
45
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA