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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EGFP-Rac1-T17N
catalog :
12982
citations: 17
Reference
Greenwich J, Alakavuklar M, Fuqua C. An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria. Microbiol Resour Announc. 2023;12:e0111922 pubmed publisher
Kitzinger R, Fritz G, Henninger C. Nuclear RAC1 is a modulator of the doxorubicin-induced DNA damage response. Biochim Biophys Acta Mol Cell Res. 2022;1869:119320 pubmed publisher
Yao Z, Qiao Y, Li X, Chen J, Ding J, Bai L, et al. Exosomes Exploit the Virus Entry Machinery and Pathway To Transmit Alpha Interferon-Induced Antiviral Activity. J Virol. 2018;92: pubmed publisher
Chang F, Kim J, Choi Y, Park K. MTA promotes chemotaxis and chemokinesis of immune cells through distinct calcium-sensing receptor signaling pathways. Biomaterials. 2018;150:14-24 pubmed publisher
Yu B, Kiechl S, Qi D, Wang X, Song Y, Weger S, et al. A Cytokine-Like Protein Dickkopf-Related Protein 3 Is Atheroprotective. Circulation. 2017;136:1022-1036 pubmed publisher
Mao M, Wang L, Chang C, Rothenberg K, Huang J, Wang Y, et al. Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection. Mol Ther. 2017;25:803-815 pubmed publisher
Teng Y, Qin H, Bahassan A, Bendzunas N, Kennedy E, Cowell J. The WASF3-NCKAP1-CYFIP1 Complex Is Essential for Breast Cancer Metastasis. Cancer Res. 2016;76:5133-42 pubmed publisher
Konopka A, Zeug A, Skupien A, Kaza B, Mueller F, Chwedorowicz A, et al. Cleavage of Hyaluronan and CD44 Adhesion Molecule Regulate Astrocyte Morphology via Rac1 Signalling. PLoS ONE. 2016;11:e0155053 pubmed publisher
Xu Q, Cao M, Song H, Chen S, Qian X, Zhao P, et al. Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization. Future Microbiol. 2016;11:1227-1248 pubmed
Alvarez Juliá A, Frasch A, Fuchsova B. Neuronal filopodium formation induced by the membrane glycoprotein M6a (Gpm6a) is facilitated by coronin-1a, Rac1, and p21-activated kinase 1 (Pak1). J Neurochem. 2016;137:46-61 pubmed publisher
Wan X, Lee M, Zhou W. Dosage-dependent role of Rac1 in podocyte injury. Am J Physiol Renal Physiol. 2016;310:F777-F784 pubmed publisher
Bentzinger C, von Maltzahn J, Dumont N, Stark D, Wang Y, Nhan K, et al. Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol. 2014;205:97-111 pubmed publisher
Rovira Clavé X, Angulo Ibáñez M, Reina M, Espel E. The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation. Cell Signal. 2014;26:1489-99 pubmed publisher
Yang S, Kim H. ROCK inhibition activates MCF-7 cells. PLoS ONE. 2014;9:e88489 pubmed publisher
Zheng L, Kim H. Low-Rac1 activity downregulates MC3T3-E1 osteoblastic cell motility on a nanoscale topography prepared on polystyrene substrates in vitro. J Biomed Mater Res A. 2013;101:1629-36 pubmed publisher
Zhang L, Tao B, Wang M, Jin H, Zhu Y. PI3K p110? isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganization. PLoS ONE. 2012;7:e44590 pubmed publisher
Subauste M, Von Herrath M, Benard V, Chamberlain C, Chuang T, Chu K, et al. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. J Biol Chem. 2000;275:9725-33 pubmed
product information
Catalog Number :
12982
Product Name :
pcDNA3-EGFP-Rac1-T17N
article :
doi10.1074/jbc.275.13.9725
id964
pubmed_id10734125
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-EGFP
backbone_mutation
backbone_originmodified pcDNA3
backbone_size6100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Rac1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7 for EGFP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
AF498964
mutationT17N Dominant negative
nameRac1 DN
shRNA_sequence
size579
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
45
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA