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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EGFP-Rac1-Q61L
catalog :
12981
citations: 25
Reference
Greenwich J, Alakavuklar M, Fuqua C. An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria. Microbiol Resour Announc. 2023;12:e0111922 pubmed publisher
Kitzinger R, Fritz G, Henninger C. Nuclear RAC1 is a modulator of the doxorubicin-induced DNA damage response. Biochim Biophys Acta Mol Cell Res. 2022;1869:119320 pubmed publisher
Zong H, Hazelbaker M, Moe C, Ems McClung S, Hu K, Walczak C. Spatial Regulation of MCAK Promotes Cell Polarization and Focal Adhesion Turnover to Drive Robust Cell Migration. Mol Biol Cell. 2021;:mbcE20050301 pubmed publisher
Venugopal S, Caggia S, Gambrell Sanders D, Khan S. Differential roles and activation of mammalian target of rapamycin complexes 1 and 2 during cell migration in prostate cancer cells. Prostate. 2020;80:412-423 pubmed publisher
Shin S, Kim K, Kim S, Kwon O, Choi C, Jeong S, et al. Exogenous 8-hydroxydeoxyguanosine ameliorates liver fibrosis through the inhibition of Rac1-NADPH oxidase signaling. J Gastroenterol Hepatol. 2020;35:1078-1087 pubmed publisher
Han R, Hu S, Qin W, Shi J, Hou Q, Wang X, et al. C3a and suPAR drive versican V1 expression in tubular cells of focal segmental glomerulosclerosis. JCI Insight. 2019;4: pubmed publisher
Mereness J, Bhattacharya S, Wang Q, Ren Y, Pryhuber G, Mariani T. Type VI collagen promotes lung epithelial cell spreading and wound-closure. PLoS ONE. 2018;13:e0209095 pubmed publisher
Caggia S, Chunduri H, Millena A, Perkins J, Venugopal S, Vo B, et al. Novel role of Giα2 in cell migration: Downstream of PI3-kinase-AKT and Rac1 in prostate cancer cells. J Cell Physiol. 2018;234:802-815 pubmed publisher
Niit M, Arulanandam R, Cass J, Geletu M, Hoskin V, Côté G, et al. Regulation of HC11 mouse breast epithelial cell differentiation by the E-cadherin/Rac axis. Exp Cell Res. 2017;361:112-125 pubmed publisher
Khan M, Salomaa S, Jacquemet G, Butt U, Miihkinen M, Deguchi T, et al. The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. J Cell Sci. 2017;130:3094-3107 pubmed publisher
Yu B, Kiechl S, Qi D, Wang X, Song Y, Weger S, et al. A Cytokine-Like Protein Dickkopf-Related Protein 3 Is Atheroprotective. Circulation. 2017;136:1022-1036 pubmed publisher
Weng N, Talbot P. The P2X7 receptor is an upstream regulator of dynamic blebbing and a pluripotency marker in human embryonic stem cells. Stem Cell Res. 2017;23:39-49 pubmed publisher
Mao M, Wang L, Chang C, Rothenberg K, Huang J, Wang Y, et al. Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection. Mol Ther. 2017;25:803-815 pubmed publisher
Li L, Chen J, Xiong G, St Clair D, Xu W, Xu R. Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture. J Cell Sci. 2017;130:190-202 pubmed publisher
Lee N, Fok K, White A, Wilson N, O Leary C, Cox H, et al. Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension. Nat Commun. 2016;7:11082 pubmed publisher
Wan X, Lee M, Zhou W. Dosage-dependent role of Rac1 in podocyte injury. Am J Physiol Renal Physiol. 2016;310:F777-F784 pubmed publisher
Zhou H, Fang J, Luo X, Zhang L, Yang J, Zhang C, et al. Downregulation of microRNA-100 enhances the ICMT-Rac1 signaling and promotes metastasis of hepatocellular carcinoma cells. Oncotarget. 2014;5:12177-88 pubmed
Mori M, Saito K, Ohta Y. ARHGAP22 localizes at endosomes and regulates actin cytoskeleton. PLoS ONE. 2014;9:e100271 pubmed publisher
Rovira Clavé X, Angulo Ibáñez M, Reina M, Espel E. The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation. Cell Signal. 2014;26:1489-99 pubmed publisher
Yang S, Kim H. ROCK inhibition activates MCF-7 cells. PLoS ONE. 2014;9:e88489 pubmed publisher
Dehapiot B, Halet G. Ran GTPase promotes oocyte polarization by regulating ERM (Ezrin/Radixin/Moesin) inactivation. Cell Cycle. 2013;12:1672-8 pubmed publisher
Dehapiot B, Carriere V, Carroll J, Halet G. Polarized Cdc42 activation promotes polar body protrusion and asymmetric division in mouse oocytes. Dev Biol. 2013;377:202-12 pubmed publisher
Zheng L, Kim H. Low-Rac1 activity downregulates MC3T3-E1 osteoblastic cell motility on a nanoscale topography prepared on polystyrene substrates in vitro. J Biomed Mater Res A. 2013;101:1629-36 pubmed publisher
Gautier Stein A, Soty M, Chilloux J, Zitoun C, Rajas F, Mithieux G. Glucotoxicity induces glucose-6-phosphatase catalytic unit expression by acting on the interaction of HIF-1? with CREB-binding protein. Diabetes. 2012;61:2451-60 pubmed
Subauste M, Von Herrath M, Benard V, Chamberlain C, Chuang T, Chu K, et al. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. J Biol Chem. 2000;275:9725-33 pubmed
product information
Catalog Number :
12981
Product Name :
pcDNA3-EGFP-Rac1-Q61L
article :
doi10.1074/jbc.275.13.9725
id964
pubmed_id10734125
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-EGFP
backbone_mutation
backbone_originInvitrogen
backbone_size6100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Rac1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7 for EGFP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
AF498964
mutationQ61L Constitutively active
nameRac1 constitutively active
shRNA_sequence
size579
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
45
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA