This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Bxb1Donor_W99A_ADRB2_LucReporter
catalog :
129789
citations: 1
Reference
Jones E, Lubock N, Venkatakrishnan A, Wang J, Tseng A, Paggi J, et al. Structural and functional characterization of G protein-coupled receptors with deep mutational scanning. elife. 2020;9: pubmed publisher
product information
Catalog Number :
129789
Product Name :
Bxb1Donor_W99A_ADRB2_LucReporter
article :
doi10.7554/eLife.54895
id28203821
pubmed_id33084570
bacterial resistance :
Kanamycin
cloning :
backbonepUC57
backbone_mutation
backbone_originGenewiz
backbone_size6350
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth strain :
encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
origin :
37
pi :
alt_names
Beta-2 Adrenergic Receptor
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterN/A
sequencing_primer_3GCACACCGGCCTTATTCC
sequencing_primer_5ttgtcgacgacggcggtct
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
geneADRB2
id154
genbank_ids
154
mutationW99A
nameADRB2
shRNA_sequence
size1314
species
9606
Homo sapiens
tags
locationN terminal on insert
tag3x FLAG
resistance markers :
3845
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA