This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT3-EF1A-MYC-IRES-lucOS
catalog :
129776
citations: 2
Reference
Zhu A, Abbas A, de Galarreta M, Guan Y, Lu S, Koeppen H, et al. Molecular correlates of clinical response and resistance to atezolizumab in combination with bevacizumab in advanced hepatocellular carcinoma. Nat Med. 2022;: pubmed publisher
Ruiz de Galarreta M, Bresnahan E, Molina Sánchez P, Lindblad K, Maier B, Sia D, et al. β-catenin activation promotes immune escape and resistance to anti-PD-1 therapy in hepatocellular carcinoma. Cancer Discov. 2019;: pubmed publisher
product information
Catalog Number :
129776
Product Name :
pT3-EF1A-MYC-IRES-lucOS
article :
doi10.1158/2159-8290.CD-19-0074
id28203890
pubmed_id31186238
bacterial resistance :
Ampicillin
cloning :
backbonepT3-EF1a
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The plasmid contains loxp sites so it s not ideal for experiments in mice expressing Cre.
growth strain :
Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
growth temp :
Slow growing plasmids, grow at least 18 hours.
origin :
37
pi :
alt_names
MYC
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1A
sequencing_primer_3
sequencing_primer_5TCAAGCCTCAGACAGTGGTTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutation
namehuman MYC
shRNA_sequence
size4100
species
9606
Homo sapiens
tags
locationC terminal on insert
tagluciferase
locationC terminal on insert
tagOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
plasmid copy :
Xin Chen (backbone)
resistance markers :
4568
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA