This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT3-EF1A-MYC-IRES-luc
catalog :
129775
citations: 4
Reference
Li X, Liang S, Fei M, Ma K, Sun L, Liu Y, et al. LncRNA CRNDE Drives the Progression of Hepatocellular Carcinoma by inducing the Immunosuppressive Niche. Int J Biol Sci. 2024;20:718-732 pubmed publisher
Ghasemi A, Martinez Usatorre A, Li L, Hicham M, Guichard A, Marcone R, et al. Cytokine-armed dendritic cell progenitors for antigen-agnostic cancer immunotherapy. Nat Cancer. 2023;: pubmed publisher
Chattopadhyay M, Jenkins E, Lechuga Vieco A, Nie K, Fiel M, Rialdi A, et al. The portrait of liver cancer is shaped by mitochondrial genetics. Cell Rep. 2022;38:110254 pubmed publisher
Ruiz de Galarreta M, Bresnahan E, Molina Sánchez P, Lindblad K, Maier B, Sia D, et al. β-catenin activation promotes immune escape and resistance to anti-PD-1 therapy in hepatocellular carcinoma. Cancer Discov. 2019;: pubmed publisher
product information
Catalog Number :
129775
Product Name :
pT3-EF1A-MYC-IRES-luc
article :
doi10.1158/2159-8290.CD-19-0074
id28203890
pubmed_id31186238
bacterial resistance :
Ampicillin
cloning :
backbonepT3-EF1a
backbone_mutationInsertion of human MYC, IRES, and firefly luciferase
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The plasmid contains loxp sites so it s not ideal for experiments in mice expressing Cre.
growth strain :
Transposon based vector that expresses human MYC followed by IRES and firefly luciferase
growth temp :
Slow growing plasmids, grow at least 18 hours.
origin :
37
pi :
alt_names
MYC
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1A
sequencing_primer_3
sequencing_primer_5TCAAGCCTCAGACAGTGGTTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutation
namehuman MYC
shRNA_sequence
size3700
species
9606
Homo sapiens
tags
locationC terminal on insert
tagluciferase
plasmid copy :
Xin Chen (backbone vector)
resistance markers :
4568
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA