This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR
catalog :
129774
citations: 1
Reference
Chuang M, Hsiao T, Tong A, Xu S, Chisholm A. DAPK interacts with Patronin and the microtubule cytoskeleton in epidermal development and wound repair. elife. 2016;5: pubmed publisher
product information
Catalog Number :
129774
Product Name :
pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR
article :
doi10.7554/eLife.15833
id28203889
pubmed_id27661253
bacterial resistance :
Ampicillin
cloning :
backbonepDEST
backbone_mutation
backbone_originInvitrogen Gateway
backbone_size2500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
Synthetic Biology
Other
Gateway
growth notes :
Please note: The plasmid region containing col-19 promoter and 5' synthetic intron are in the opposite orientation in the plasmid compared to the depositor's sequence. This is not known to be a concern for plasmid function.
growth strain :
Marker of microtubule dynamics in C. elegans hypodermis
origin :
37
pi :
alt_names
col-19p
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5HindIII
promoterPcol-19
sequencing_primer_3ttgatgaactgatgtctttc
sequencing_primer_5CAGGAAACAGCTATGACCATG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namePcol-19
shRNA_sequence
size665
species
6239
Caenorhabditis elegans
tags
locationN terminal on backbone
tagSynthetic Intron
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3CTGAACTTGTGGCCGTTTAC
sequencing_primer_5CCAAAGGACCCAAAGGTATG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_VW02B12L.3
geneebp-2
id174744
genbank_ids
CELE_VW02B12L.3
mutation
nameebp-2
shRNA_sequence
size1100
species
6239
Caenorhabditis elegans
tags
locationC terminal on backbone
tagmGFP
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3TGCCGCATAGTTAAGCCAGC
sequencing_primer_5TCGGCATGGACGAGCTGTAC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameunc-54 3'UTR
shRNA_sequence
size735
species
6239
Caenorhabditis elegans
tags
plasmid copy :
Made by Suhong Xu in the Chisholm Lab
resistance markers :
2758
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA