This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ORF31-2xStrep pCDNA4
catalog :
129744
citations: 4
Reference
McCollum C, Didychuk A, Liu D, Murray Nerger L, Cristea I, Glaunsinger B. The viral packaging motor potentiates Kaposi's sarcoma-associated herpesvirus gene expression late in infection. PLoS Pathog. 2023;19:e1011163 pubmed publisher
Castañeda A, Didychuk A, Louder R, McCollum C, Davis Z, Nogales E, et al. The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription. PLoS Pathog. 2020;16:e1008843 pubmed publisher
Didychuk A, Castañeda A, Kushnir L, Huang C, Glaunsinger B. Conserved CxnC motifs in Kaposi's sarcoma-associated herpesvirus ORF66 are required for viral late gene expression and are essential for its interaction with ORF34. J Virol. 2019;: pubmed publisher
Castañeda A, Glaunsinger B. The Interaction between ORF18 and ORF30 Is Required for Late Gene Expression in Kaposi's Sarcoma-Associated Herpesvirus. J Virol. 2019;93: pubmed publisher
product information
Catalog Number :
129744
Product Name :
ORF31-2xStrep pCDNA4
article :
doi10.1128/JVI.01488-18
id28197074
pubmed_id30305361
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA4/TO
backbone_mutation
backbone_origin
backbone_size5168
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHV8GK18_gp35
geneORF31
id4961498
genbank_ids
4961498
mutation
nameORF31
shRNA_sequence
size673
species
32644
Other
Kaposi's sarcoma-associated herpesvirus (HHV-8)
tags
locationC terminal on backbone
tag2xStrep Tag II
resistance markers :
4579
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA