This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
catalog :
129717
citations: 4
Reference
Chen Y, Ying Y, Ma W, Ma H, Shi L, Gao X, et al. Targeting the epigenetic reader ENL inhibits super-enhancer-driven oncogenic transcription and synergizes with BET inhibition to suppress tumor progression. Cancer Res. 2024;: pubmed publisher
Li J, Dai C, Xie W, Zhang H, Huang X, Chronis C, et al. A One-step strategy to target essential factors with auxin-inducible degron system in mouse embryonic stem cells. Front Cell Dev Biol. 2022;10:964119 pubmed publisher
Yunusova A, Smirnov A, Shnaider T, Lukyanchikova V, Afonnikova S, Battulin N. Evaluation of the OsTIR1 and AtAFB2 AID Systems for Genome Architectural Protein Degradation in Mammalian Cells. Front Mol Biosci. 2021;8:757394 pubmed publisher
Li S, Prasanna X, Salo V, Vattulainen I, Ikonen E. An efficient auxin-inducible degron system with low basal degradation in human cells. Nat Methods. 2019;16:866-869 pubmed publisher
product information
Catalog Number :
129717
Product Name :
pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
article :
doi10.1038/s41592-019-0512-x
id28203879
pubmed_id31451765
bacterial resistance :
Ampicillin
cloning :
backbonepSH-EFIRES-P
backbone_mutation
backbone_origin
backbone_size8200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
TALEN
Other
Human safe harbor locus (A AVS1) site-specific integration
growth strain :
AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS
origin :
37
pi :
alt_names
auxin signaling F-box 2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BglII
promoterEF1a
sequencing_primer_3acaccggccttattccaagc
sequencing_primer_5tctctccacaggtgtccact
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G26810, auxin signaling F-box 2
geneAFB2
id822296
genbank_ids
mutationcodon optimized
nameAFB2
shRNA_sequence
size2472
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmCherry-weak NLS
plasmid copy :
AtAFB2 codon-optimized and synthesized by Genscript.
resistance markers :
3281
tags :
High Copy
terms :
Puromycin
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA