This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSH-EFIRES-P-AtAFB2
catalog :
129715
citations: 4
Reference
Fung H, Hayashi Y, Salo V, Babenko A, Zagoriy I, Brunner A, et al. Genetically encoded multimeric tags for subcellular protein localization in cryo-EM. Nat Methods. 2023;20:1900-1908 pubmed publisher
Takahashi K, Kanerva K, Vanharanta L, Almeida Souza L, Lietha D, Olkkonen V, et al. ORP2 couples LDL-cholesterol transport to FAK activation by endosomal cholesterol/PI(4,5)P2 exchange. EMBO J. 2021;40:e106871 pubmed publisher
Vanharanta L, Peranen J, Pfisterer S, Enkavi G, Vattulainen I, Ikonen E. High-content imaging and structure-based predictions reveal functional differences between Niemann-Pick C1 variants. Traffic. 2020;21:386-397 pubmed publisher
Li S, Prasanna X, Salo V, Vattulainen I, Ikonen E. An efficient auxin-inducible degron system with low basal degradation in human cells. Nat Methods. 2019;16:866-869 pubmed publisher
product information
Catalog Number :
129715
Product Name :
pSH-EFIRES-P-AtAFB2
article :
doi10.1038/s41592-019-0512-x
id28203879
pubmed_id31451765
bacterial resistance :
Ampicillin
cloning :
backbonepSH-EFIRES-P
backbone_mutation
backbone_origin
backbone_size8200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
TALEN
Other
Human safe harbor locus (A AVS1) site-specific integration
growth strain :
AAVS1 safe harbor targeting vector expressing AtAFB2
origin :
37
pi :
alt_names
auxin signaling F-box 2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BglII
promoterEF1a
sequencing_primer_3acaccggccttattccaagc
sequencing_primer_5tctctccacaggtgtccact
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G26810, auxin signaling F-box 2
geneAFB2
id822296
genbank_ids
mutationcodon optimized
nameAFB2
shRNA_sequence
size1728
species
3702
Arabidopsis thaliana
tags
location
tagno
plasmid copy :
AtAFB2 codon-optimized and synthesized by Genscript.
resistance markers :
3281
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA