This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EGFP-RhoA-Q63L
catalog :
12968
citations: 29
Reference
Yue X, Cui J, Sun Z, Liu L, Li Y, Shao L, et al. Nuclear softening mediated by Sun2 suppression delays mechanical stress-induced cellular senescence. Cell Death Discov. 2023;9:167 pubmed publisher
Zanin J, Friedman W. p75NTR prevents the onset of cerebellar granule cell migration via RhoA activation. elife. 2022;11: pubmed publisher
Kumar R, Francis V, Kulasekaran G, Khan M, Armstrong G, McPherson P. A cell-based GEF assay reveals new substrates for DENN domains and a role for DENND2B in primary ciliogenesis. Sci Adv. 2022;8:eabk3088 pubmed publisher
Finney A, Scott M, Reeves K, Wang D, Alfaidi M, Schwartz J, et al. EphA2 signaling within integrin adhesions regulates fibrillar adhesion elongation and fibronectin deposition. Matrix Biol. 2021;103-104:1-21 pubmed publisher
Sala S, Oakes P. Stress fiber strain recognition by the LIM protein testin is cryptic and mediated by RhoA. Mol Biol Cell. 2021;:mbcE21030156 pubmed publisher
Wu D, Harrison D, Szasz T, Yeh C, Shentu T, Meliton A, et al. Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells. Nat Metab. 2021;3:714-727 pubmed publisher
McCray B, Diehl E, Sullivan J, Aisenberg W, Zaccor N, Lau A, et al. Neuropathy-causing TRPV4 mutations disrupt TRPV4-RhoA interactions and impair neurite extension. Nat Commun. 2021;12:1444 pubmed publisher
Priya R, Allanki S, Gentile A, Mansingh S, Uribe V, Maischein H, et al. Tension heterogeneity directs form and fate to pattern the myocardial wall. Nature. 2020;588:130-134 pubmed publisher
Townshend R, Shao Y, Wang S, Cortez C, Esfahani S, Spence J, et al. Effect of Cell Spreading on Rosette Formation by Human Pluripotent Stem Cell-Derived Neural Progenitor Cells. Front Cell Dev Biol. 2020;8:588941 pubmed publisher
Budnar S, Husain K, Gomez G, Naghibosadat M, Varma A, Verma S, et al. Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA Residence Kinetics. Dev Cell. 2019;: pubmed publisher
Hu X, Liu Z, Chen X, Schulz V, Kumar A, Hartman A, et al. MKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation. Nat Commun. 2019;10:1695 pubmed publisher
Cortés E, Sarper M, Robinson B, Lachowski D, Chronopoulos A, Thorpe S, et al. GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment. EMBO Rep. 2019;20: pubmed publisher
Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed publisher
O Loughlin T, Masters T, Buss F. The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics. EMBO Rep. 2018;19: pubmed publisher
Singh R, Haka A, Brumfield A, Grosheva I, Bhardwaj P, Chin H, et al. Ceramide activation of RhoA/Rho kinase impairs actin polymerization during aggregated LDL catabolism. J Lipid Res. 2017;58:1977-1987 pubmed publisher
Yu B, Kiechl S, Qi D, Wang X, Song Y, Weger S, et al. A Cytokine-Like Protein Dickkopf-Related Protein 3 Is Atheroprotective. Circulation. 2017;136:1022-1036 pubmed publisher
Nardone G, Oliver De La Cruz J, Vrbsky J, Martini C, Pribyl J, Skladal P, et al. YAP regulates cell mechanics by controlling focal adhesion assembly. Nat Commun. 2017;8:15321 pubmed publisher
Yuan X, Cao J, He X, Serra R, Qu J, Cao X, et al. Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation. Nat Commun. 2016;7:11024 pubmed publisher
Alvarez Juliá A, Frasch A, Fuchsova B. Neuronal filopodium formation induced by the membrane glycoprotein M6a (Gpm6a) is facilitated by coronin-1a, Rac1, and p21-activated kinase 1 (Pak1). J Neurochem. 2016;137:46-61 pubmed publisher
Yeh C, Hsu L. Zebrafish diras1 Promoted Neurite Outgrowth in Neuro-2a Cells and Maintained Trigeminal Ganglion Neurons In Vivo via Rac1-Dependent Pathway. Mol Neurobiol. 2016;53:6594-6607 pubmed
Yu C, Rafiq N, Cao F, Zhou Y, Krishnasamy A, Biswas K, et al. Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force. Nat Commun. 2015;6:8672 pubmed publisher
Wong S, Mosabbir A, Truong K. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE. 2015;10:e0135965 pubmed publisher
Wong A, Loots G, Yellowley C, Dosé A, Genetos D. Parathyroid hormone regulation of hypoxia-inducible factor signaling in osteoblastic cells. Bone. 2015;81:97-103 pubmed publisher
Rovira Clavé X, Angulo Ibáñez M, Reina M, Espel E. The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation. Cell Signal. 2014;26:1489-99 pubmed publisher
Yang S, Kim H. ROCK inhibition activates MCF-7 cells. PLoS ONE. 2014;9:e88489 pubmed publisher
Palomero T, Couronné L, Khiabanian H, Kim M, Ambesi Impiombato A, Perez Garcia A, et al. Recurrent mutations in epigenetic regulators, RHOA and FYN kinase in peripheral T cell lymphomas. Nat Genet. 2014;46:166-70 pubmed publisher
Kong Y, Carrion B, Singh R, Putnam A. Matrix identity and tractional forces influence indirect cardiac reprogramming. Sci Rep. 2013;3:3474 pubmed publisher
Wong S, Mills E, Truong K. Simultaneous assembly of two target proteins using split inteins for live cell imaging. Protein Eng Des Sel. 2013;26:207-13 pubmed publisher
Subauste M, Von Herrath M, Benard V, Chamberlain C, Chuang T, Chu K, et al. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. J Biol Chem. 2000;275:9725-33 pubmed
product information
Catalog Number :
12968
Product Name :
pcDNA3-EGFP-RhoA-Q63L
article :
doi10.1074/jbc.275.13.9725
id964
pubmed_id10734125
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-EGFP
backbone_mutation
backbone_originInvitrogen
backbone_size6100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
RhoA
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7 for EGFP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
L25080
mutationQ63L Constitutively active
nameRhoA constitutively active
shRNA_sequence
size582
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
45
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA