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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EGFP-RhoA-T19N
catalog :
12967
citations: 26
Reference
Kumar R, Francis V, Kulasekaran G, Khan M, Armstrong G, McPherson P. A cell-based GEF assay reveals new substrates for DENN domains and a role for DENND2B in primary ciliogenesis. Sci Adv. 2022;8:eabk3088 pubmed publisher
Finney A, Scott M, Reeves K, Wang D, Alfaidi M, Schwartz J, et al. EphA2 signaling within integrin adhesions regulates fibrillar adhesion elongation and fibronectin deposition. Matrix Biol. 2021;103-104:1-21 pubmed publisher
Sala S, Oakes P. Stress fiber strain recognition by the LIM protein testin is cryptic and mediated by RhoA. Mol Biol Cell. 2021;:mbcE21030156 pubmed publisher
Wu D, Harrison D, Szasz T, Yeh C, Shentu T, Meliton A, et al. Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells. Nat Metab. 2021;3:714-727 pubmed publisher
McCray B, Diehl E, Sullivan J, Aisenberg W, Zaccor N, Lau A, et al. Neuropathy-causing TRPV4 mutations disrupt TRPV4-RhoA interactions and impair neurite extension. Nat Commun. 2021;12:1444 pubmed publisher
Priya R, Allanki S, Gentile A, Mansingh S, Uribe V, Maischein H, et al. Tension heterogeneity directs form and fate to pattern the myocardial wall. Nature. 2020;588:130-134 pubmed publisher
Hung P, Huang M, Kuo Y, Yang J. The Inhibition of Wnt Restrain KRASG12V-Driven Metastasis in Non-Small-Cell Lung Cancer. Cancers (Basel). 2020;12: pubmed publisher
Shen W, Kilander M, Bridi M, Frei J, Niescier R, Huang S, et al. Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors. J Neurosci Res. 2020;98:1213-1231 pubmed publisher
Fray M, Charpentier J, Sylvain N, Seminario M, Bunnell S. Vav2 lacks Ca2+ entry-promoting scaffolding functions unique to Vav1 and inhibits T cell activation via Cdc42. J Cell Sci. 2020;133: pubmed publisher
Hu X, Liu Z, Chen X, Schulz V, Kumar A, Hartman A, et al. MKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation. Nat Commun. 2019;10:1695 pubmed publisher
Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed publisher
Fox M, Chandra R, Menken M, Larkin E, Nam H, Engeln M, et al. Dendritic remodeling of D1 neurons by RhoA/Rho-kinase mediates depression-like behavior. Mol Psychiatry. 2018;: pubmed publisher
Yu B, Kiechl S, Qi D, Wang X, Song Y, Weger S, et al. A Cytokine-Like Protein Dickkopf-Related Protein 3 Is Atheroprotective. Circulation. 2017;136:1022-1036 pubmed publisher
Shah M, Garcia Pak I, Darling E. Influence of Inherent Mechanophenotype on Competitive Cellular Adherence. Ann Biomed Eng. 2017;45:2036-2047 pubmed publisher
Rafiq N, Lieu Z, Jiang T, Yu C, Matsudaira P, Jones G, et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol. 2017;216:181-197 pubmed publisher
Gorbunova E, Simons M, Gavrilovskaya I, Mackow E. The Andes Virus Nucleocapsid Protein Directs Basal Endothelial Cell Permeability by Activating RhoA. MBio. 2016;7: pubmed publisher
Yuan X, Cao J, He X, Serra R, Qu J, Cao X, et al. Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation. Nat Commun. 2016;7:11024 pubmed publisher
Xu Q, Cao M, Song H, Chen S, Qian X, Zhao P, et al. Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization. Future Microbiol. 2016;11:1227-1248 pubmed
Wheeler D, Underhill S, Stolz D, Murdoch G, Thiels E, Romero G, et al. Amphetamine activates Rho GTPase signaling to mediate dopamine transporter internalization and acute behavioral effects of amphetamine. Proc Natl Acad Sci U S A. 2015;112:E7138-47 pubmed publisher
Ibeawuchi S, Agbor L, Quelle F, Sigmund C. Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors. J Biol Chem. 2015;290:19208-17 pubmed publisher
Bentzinger C, von Maltzahn J, Dumont N, Stark D, Wang Y, Nhan K, et al. Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol. 2014;205:97-111 pubmed publisher
Rovira Clavé X, Angulo Ibáñez M, Reina M, Espel E. The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation. Cell Signal. 2014;26:1489-99 pubmed publisher
Yang S, Kim H. ROCK inhibition activates MCF-7 cells. PLoS ONE. 2014;9:e88489 pubmed publisher
Kong Y, Carrion B, Singh R, Putnam A. Matrix identity and tractional forces influence indirect cardiac reprogramming. Sci Rep. 2013;3:3474 pubmed publisher
Gozo M, Aspuria P, Cheon D, Walts A, Berel D, Miura N, et al. Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis. Cell Death Differ. 2013;20:1031-42 pubmed publisher
Subauste M, Von Herrath M, Benard V, Chamberlain C, Chuang T, Chu K, et al. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. J Biol Chem. 2000;275:9725-33 pubmed
product information
Catalog Number :
12967
Product Name :
pcDNA3-EGFP-RhoA-T19N
article :
doi10.1074/jbc.275.13.9725
id964
pubmed_id10734125
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-EGFP
backbone_mutation
backbone_originInvitrogen
backbone_size6100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
RhoA
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7 for EGFP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
L25080
mutationT19N Dominant negative
nameRhoA DN
shRNA_sequence
size582
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
45
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA