This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EGFP-RhoA-wt
catalog :
12965
citations: 21
Reference
Finney A, Scott M, Reeves K, Wang D, Alfaidi M, Schwartz J, et al. EphA2 signaling within integrin adhesions regulates fibrillar adhesion elongation and fibronectin deposition. Matrix Biol. 2021;103-104:1-21 pubmed publisher
Wu D, Harrison D, Szasz T, Yeh C, Shentu T, Meliton A, et al. Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells. Nat Metab. 2021;3:714-727 pubmed publisher
McCray B, Diehl E, Sullivan J, Aisenberg W, Zaccor N, Lau A, et al. Neuropathy-causing TRPV4 mutations disrupt TRPV4-RhoA interactions and impair neurite extension. Nat Commun. 2021;12:1444 pubmed publisher
Townshend R, Shao Y, Wang S, Cortez C, Esfahani S, Spence J, et al. Effect of Cell Spreading on Rosette Formation by Human Pluripotent Stem Cell-Derived Neural Progenitor Cells. Front Cell Dev Biol. 2020;8:588941 pubmed publisher
Shen W, Kilander M, Bridi M, Frei J, Niescier R, Huang S, et al. Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors. J Neurosci Res. 2020;98:1213-1231 pubmed publisher
Dupraz S, Hilton B, Husch A, Santos T, Coles C, Stern S, et al. RhoA Controls Axon Extension Independent of Specification in the Developing Brain. Curr Biol. 2019;29:3874-3886.e9 pubmed publisher
Budnar S, Husain K, Gomez G, Naghibosadat M, Varma A, Verma S, et al. Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA Residence Kinetics. Dev Cell. 2019;: pubmed publisher
Hu X, Liu Z, Chen X, Schulz V, Kumar A, Hartman A, et al. MKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation. Nat Commun. 2019;10:1695 pubmed publisher
Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed publisher
Fox M, Chandra R, Menken M, Larkin E, Nam H, Engeln M, et al. Dendritic remodeling of D1 neurons by RhoA/Rho-kinase mediates depression-like behavior. Mol Psychiatry. 2018;: pubmed publisher
Cortes J, Ambesi Impiombato A, Couronné L, Quinn S, Kim C, da Silva Almeida A, et al. RHOA G17V Induces T Follicular Helper Cell Specification and Promotes Lymphomagenesis. Cancer Cell. 2018;33:259-273.e7 pubmed publisher
Egami Y, Kawai K, Araki N. RhoC regulates the actin remodeling required for phagosome formation during Fc?R-mediated phagocytosis. J Cell Sci. 2017;130:4168-4179 pubmed publisher
Singh R, Haka A, Brumfield A, Grosheva I, Bhardwaj P, Chin H, et al. Ceramide activation of RhoA/Rho kinase impairs actin polymerization during aggregated LDL catabolism. J Lipid Res. 2017;58:1977-1987 pubmed publisher
Feng J, Yang H, Zhang Y, Wei H, Zhu Z, Zhu B, et al. Tumor cell-derived lactate induces TAZ-dependent upregulation of PD-L1 through GPR81 in human lung cancer cells. Oncogene. 2017;36:5829-5839 pubmed publisher
Priya R, Liang X, Teo J, Duszyc K, Yap A, Gomez G. ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens. Mol Biol Cell. 2017;28:12-20 pubmed publisher
Yuan X, Cao J, He X, Serra R, Qu J, Cao X, et al. Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation. Nat Commun. 2016;7:11024 pubmed publisher
Xu Q, Cao M, Song H, Chen S, Qian X, Zhao P, et al. Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization. Future Microbiol. 2016;11:1227-1248 pubmed
Ibeawuchi S, Agbor L, Quelle F, Sigmund C. Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors. J Biol Chem. 2015;290:19208-17 pubmed publisher
Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, et al. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. FASEB J. 2014;28:1938-46 pubmed publisher
Xing L, Yao X, Williams K, Bassell G. Negative regulation of RhoA translation and signaling by hnRNP-Q1 affects cellular morphogenesis. Mol Biol Cell. 2012;23:1500-9 pubmed publisher
Subauste M, Von Herrath M, Benard V, Chamberlain C, Chuang T, Chu K, et al. Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. J Biol Chem. 2000;275:9725-33 pubmed
product information
Catalog Number :
12965
Product Name :
pcDNA3-EGFP-RhoA-wt
article :
doi10.1074/jbc.275.13.9725
id964
pubmed_id10734125
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3-EGFP
backbone_mutation
backbone_originInvitrogen
backbone_size6100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7 for EGFP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
L25080
mutation
nameRhoA
shRNA_sequence
size582
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
45
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA