This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFB1_hMSH2
catalog :
129423
citations: 2
Reference
Chen P, Hussmann J, Yan J, Knipping F, Ravisankar P, Chen P, et al. Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell. 2021;184:5635-5652.e29 pubmed publisher
Geng H, Du C, Chen S, Salerno V, Manfredi C, Hsieh P. In vitro studies of DNA mismatch repair proteins. Anal Biochem. 2011;413:179-84 pubmed publisher
product information
Catalog Number :
129423
Product Name :
pFB1_hMSH2
article :
doi10.1016/j.ab.2011.02.017
id16303
pubmed_id21329650
bacterial resistance :
Ampicillin
cloning :
backbonepFastBac 1
backbone_mutationhMSH2 is inserted in MCS (5'- BamH 1 and 3'- Xho I) of pFastBac 1. 5' sequencing primer covers nucleotides from 120-151 of hMSH2, but antiparallel, 3' sequencing primer covers nucleotides from 2655-2685 of hMSH2. They are used to sequence junction of the insert. The plasmid is used to amplify and generate recombinant bacmid DNA. which will then be transfected into insect cells for virus generation and eventually protein (hMSH2) expression.
backbone_originInvitrogen
backbone_size4776
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Expression human MSH2 protein using baculovirus
growth temp :
Use DH10Bac for bacmid expression
origin :
37
pi :
alt_names
DNA mismatch repair protein homolog (MSH2)
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho I
cloning_site_5BamH I
promoterPolyhedrin promoter
sequencing_primer_3ggagttcctgtccaaggtgaaacaaatgccc
sequencing_primer_5gcgcgtcctcgccgtgcgccgtatagaagtcg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCOCA1, FCC1, HNPCC, HNPCC1, LCFS2, LYNCH1, MMRCS2, MSH-2, hMSH2
geneMSH2
id4436
genbank_ids
BC021566
mutation
namehuman MSH2
shRNA_sequence
size2805
species
9606
Homo sapiens
tags
resistance markers :
210
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA