pCag FlpO-2A-Cre EV | Addgene 129419 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCag FlpO-2A-Cre EV

catalog :

129419

citations: 2

Reference
Ayala Sarmiento A, Kobritz N, Breunig J. De Novo Generation of Murine and Human MADR Recipient Cell Lines for Locus-Specific, Stable Integration of Transgenic Elements. STAR Protoc. 2020;1:100184 pubmed publisher
Kim G, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra Clarke M, et al. Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Cell. 2019;179:251-267.e24 pubmed publisher

product information

Catalog Number :

129419

Product Name :

pCag FlpO-2A-Cre EV

article :

doi	10.1016/j.cell.2019.08.013
id	28203812
pubmed_id	31539496

bacterial resistance :

Ampicillin

cloning :

backbone

pOG44

backbone_mutation

CMV promoter converted to CAG promoter

backbone_origin

ThermoFisher

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Cre/Lox

Other

Unspecified

Episomal expression vector

growth strain :

episomal expression of FlpO and Cre recombinases

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV/Chick -actin (CAG)
sequencing_primer_3
sequencing_primer_5	pCAG-F
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

FlpO-2A-Cre

shRNA_sequence

size

2364

species

4932

Saccharomyces cerevisiae

32644

Other

P1 bacteriophage