This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV 3xFLAG-LC3B Q116P G120
catalog :
129289
citations: 1
Reference
Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R. Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. J Biol Chem. 2019;294:12610-12621 pubmed publisher
product information
Catalog Number :
129289
Product Name :
pCMV 3xFLAG-LC3B Q116P G120
article :
doi10.1074/jbc.AC119.009977
id28203798
pubmed_id31315929
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-tripleFLAG-Gateway
backbone_mutation
backbone_origin
backbone_size4711
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3hGH-PA-R
sequencing_primer_5CMVF_pCDNA3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
NM_022818.4
mutationChanged Glutamine 116 to Proline. Deleted amino acids 121-125 (stop codon after G120).
nameMicrotubule-associated proteins 1A/1B light chain 3B
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xFLAG
resistance markers :
2021
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA