This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T-1-3xMyc-ERK2(K52R)
catalog :
129228
citations: 1
Reference
Smolko C, Janes K. An ultrasensitive fiveplex activity assay for cellular kinases. Sci Rep. 2019;9:19409 pubmed publisher
product information
Catalog Number :
129228
Product Name :
pGEX-4T-1-3xMyc-ERK2(K52R)
article :
doi10.1038/s41598-019-55998-8
id28203788
pubmed_id31857650
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T1
backbone_mutation
backbone_originAmersham
backbone_size4969
promoter
sequencing_primer_33GEX
sequencing_primer_55GEX
vector_types
Bacterial Expression
growth notes :
Depositor uses BL21 for protein production.
growth strain :
Encodes Myc tagged human ERK2(K52R) for purification by GST affinity
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_33GEX
sequencing_primer_55GEX
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesERK-2, ERT1, Erk2, p42-MAPK
geneMapk1
id116590
genbank_ids
NM_053842
mutationChanged Lysine 52 to Arginine
nameERK2(K52R)
shRNA_sequence
size1179
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagGST
locationN terminal on backbone
tagMyc
resistance markers :
1585
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA