This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-29b-3xGluGlu-c-jun-His6
catalog :
129227
citations: 2
Reference
Broche J, K xf6 hler A, K xfc hnel F, Osteresch B, Chandrasekaran T, Adam S, et al. Genome-wide deposition of 6-methyladenine in human DNA reduces the viability of HEK293 cells and directly influences gene expression. Commun Biol. 2023;6:138 pubmed publisher
Smolko C, Janes K. An ultrasensitive fiveplex activity assay for cellular kinases. Sci Rep. 2019;9:19409 pubmed publisher
product information
Catalog Number :
129227
Product Name :
pET-29b-3xGluGlu-c-jun-His6
article :
doi10.1038/s41598-019-55998-8
id28203788
pubmed_id31857650
bacterial resistance :
Kanamycin
cloning :
backbonepET 29b
backbone_mutation
backbone_originEMD Biosciences
backbone_size5370
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Depositor uses BL21 for protein production.
growth strain :
Encodes 3xGluGlu tagged human c-Jun for purification by nickel affinity
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SacI
cloning_site_5NcoI
promoter
sequencing_primer_3T7 Term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAP-1, AP1, c-Jun, cJUN, p39
geneJUN
id3725
genbank_ids
NM_002228
mutation
namecJun
shRNA_sequence
size1058
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag6xHistidine
resistance markers :
1585
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA