pGEX-4T-1-3xHA-I B (1-62) | Addgene 129226 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEX-4T-1-3xHA-I B (1-62)

catalog :

129226

citations: 1

Reference
Smolko C, Janes K. An ultrasensitive fiveplex activity assay for cellular kinases. Sci Rep. 2019;9:19409 pubmed publisher

product information

Catalog Number :

129226

Product Name :

pGEX-4T-1-3xHA-I B (1-62)

article :

doi	10.1038/s41598-019-55998-8
id	28203788
pubmed_id	31857650

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX-4T1

backbone_mutation

backbone_origin

Amersham

backbone_size

4969

promoter

sequencing_primer_3

3GEX

sequencing_primer_5

5GEX

vector_types

Bacterial Expression

growth notes :

Depositor uses BL21 for protein production. There is a single mutation in LacI- L189S, but this does not affect plasmid function.

growth strain :

Encodes HA tagged human I B (1-62) for purification by GST affinity

origin :

pi :

alt_names

NFKBIA

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3	3GEX
sequencing_primer_5	5GEX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	EDAID2, IKBA, MAD-3, NFKBI
gene	NFKBIA
id	4792

genbank_ids

NM_020529

mutation

name

IkBa(1-62)

shRNA_sequence

size

201

species

9606

Homo sapiens