pGAN201 | Addgene 129204 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGAN201

catalog :

129204

citations: 1

Reference
Newby G, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso C, et al. A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance. Cell. 2017;171:966-979.e18 pubmed publisher

product information

Catalog Number :

129204

Product Name :

pGAN201

article :

doi	10.1016/j.cell.2017.09.041
id	28203780
pubmed_id	29056345

bacterial resistance :

Ampicillin

cloning :

backbone

pGAN147

backbone_mutation

LR reaction with pENTR-Rnq1

backbone_origin

Gregory Newby

backbone_size

8600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

Synthetic Biology

growth notes :

This is the basic yTRAP sensor to sense aggregation of Rnq1 or [RNQ+]. It should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.

growth strain :

yTRAP sensor of [RNQ+], detects aggregation of Rnq1

origin :

pi :

alt_names

YCL028W

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	SUP35
sequencing_primer_3	Unknown
sequencing_primer_5	Unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YCL028W
gene	RNQ1
id	850329

genbank_ids

NM_001178673.1

mutation

name

RNQ1

shRNA_sequence

size

4000

species

4932

Saccharomyces cerevisiae

100

Synthetic