This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGK-Ptrf-mKate2
catalog :
128765
citations: 1
Reference
Stone N, Gifford C, Thomas R, Pratt K, Samse Knapp K, Mohamed T, et al. Context-Specific Transcription Factor Functions Regulate Epigenomic and Transcriptional Dynamics during Cardiac Reprogramming. Cell Stem Cell. 2019;25:87-102.e9 pubmed publisher
product information
Catalog Number :
128765
Product Name :
pGK-Ptrf-mKate2
article :
doi10.1016/j.stem.2019.06.012
id28203713
pubmed_id31271750
bacterial resistance :
Ampicillin
cloning :
backbonepGK:HygroR-CMV:mKate2-B_UTR Addgene #68480
backbone_mutationPtrf regulatory sequence (chr11:100,830,367-100,831,115) was inserted into pGK:HygroR-CMV:mKate2-B_UTR (a gift from Tyler Jacks; Addgene #68480), in place of CMV, after excision with AfeI and AvrII (NEB).
backbone_originAddgene
backbone_size8164
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/642900v1 for bioRxiv preprint.
growth strain :
Fluorescent mKate reporter for Ptrf
origin :
37
pi :
alt_names
Cavin1; Cavin; Cav-p60; AW546441; 2310075E07Rik
ENSMUSG00000004044
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterPtrf
sequencing_primer_3CCACTATCGGCGAGTACTTCTA
sequencing_primer_5CCGTGAACAACCACCACTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases2310075E07Rik, Cav-p60, Cavin, Ptrf
geneCavin1
id19285
genbank_ids
mutation
namePtrf promoter
shRNA_sequence
size749
species
10090
Mus musculus
tags
resistance markers :
4532
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA