This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCVhyg-H3.3WT-FLAG/HA
catalog :
128740
citations: 2
Reference
Tsukahara T, Kethireddy S, Bonefas K, Chen A, Sutton B, Dou Y, et al. Division of labor among H3K4 Methyltransferases Defines Distinct Facets of Homeostatic Plasticity. bioRxiv. 2023;: pubmed publisher
Jang Y, Broun A, Wang C, Park Y, Zhuang L, Lee J, et al. H3.3K4M destabilizes enhancer H3K4 methyltransferases MLL3/MLL4 and impairs adipose tissue development. Nucleic Acids Res. 2019;47:607-620 pubmed publisher
product information
Catalog Number :
128740
Product Name :
pMSCVhyg-H3.3WT-FLAG/HA
article :
doi10.1093/nar/gky982
id28203702
pubmed_id30335158
bacterial resistance :
Ampicillin
cloning :
backbonepMSCVhyg
backbone_mutation
backbone_originClontech
backbone_size553
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth strain :
Expression of histone H3.3WT-FLAG/HA
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HpaI
cloning_site_5XhoI
promoterMSCV
sequencing_primer_3GTGCGAGGCCAGAGGCCA
sequencing_primer_5CTTCTCTAGGCGCCGGAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRYLIB1, H3-3B, H3.3A, H3F3, H3F3A
geneH3-3A
id3020
genbank_ids
NM_002107.6
mutation
nameH3.3 WT
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFLAG and HA
resistance markers :
159
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA