pPHAGE-CR-PINK1-C-TAP | Addgene 128510 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pPHAGE-CR-PINK1-C-TAP

catalog :

128510

citations: 1

Reference
Moutaoufik M, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, et al. Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. iScience. 2019;19:1114-1132 pubmed publisher

product information

Catalog Number :

128510

Product Name :

pPHAGE-CR-PINK1-C-TAP

article :

doi	10.1016/j.isci.2019.08.057
id	28203664
pubmed_id	31536960

bacterial resistance :

Ampicillin

cloning :

backbone

pPHAGE C-TAP

backbone_mutation

backbone_origin

backbone_size

10056

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	AAGAAGCGGAGACGGTTAGG
sequencing_primer_5	CMV-F CGCAAATGGGCGGTAGGCGTG, ATCCACGCTGTTTTGACCTC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BRPK, PARK6
gene	PINK1
id	65018

genbank_ids

mutation

Synonymous silent mutation in Q5 to render sgRNA PAM site silent

name

PTEN induced kinase 1

shRNA_sequence

size

species

9606

Homo sapiens