This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPHAGE-C20orf24- C20orf24-3 UTR-VAR
catalog :
128509
citations: 1
Reference
Moutaoufik M, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, et al. Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis. iScience. 2019;19:1114-1132 pubmed publisher
product information
Catalog Number :
128509
Product Name :
pPHAGE-C20orf24- C20orf24-3 UTR-VAR
article :
doi10.1016/j.isci.2019.08.057
id28203664
pubmed_id31536960
bacterial resistance :
Ampicillin
cloning :
backbonepPHAGE C-TAP
backbone_mutationAdded 3'UTR of C20orf24 with c.*398G>A (NM_018840.5) downstream from C20orf24 CDS
backbone_origin
backbone_size10056
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Please note: Plasmid contains a P136L mutation in C20orf24 compared to the NCBI reference squence NP_001186463.1. This mutation is not known to affect plasmid function.
growth strain :
Lentiviral constitutive expression of C20orf24 under control of its native 3'UTR of human C20orf24 with variant from COX deficiency patient.
origin :
30
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3IRES-R CCTCACATTGCCAAAAGACG
sequencing_primer_5GAGGATACCCCTACGACGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC20orf24, PNAS-11, RIP5
geneRAB5IF
id55969
genbank_ids
NM_018840.5
mutation
nameRAB5IF
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFLAG and HA tags
resistance markers :
3055
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA