This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCri-H6-TEV-GRAB-STREP
catalog :
128499
citations: 1
Reference
Marino Puertas L, Del Amo Maestro L, Taulés M, Gomis Ruth F, Goulas T. Recombinant production of human α2-macroglobulin variants and interaction studies with recombinant G-related α2-macroglobulin binding protein and latent transforming growth factor-β2. Sci Rep. 2019;9:9186 pubmed publisher
product information
Catalog Number :
128499
Product Name :
pCri-H6-TEV-GRAB-STREP
article :
doi10.1038/s41598-019-45712-z
id28203662
pubmed_id31235767
bacterial resistance :
Kanamycin
cloning :
backbonepCri8a
backbone_mutationIntroduction of STREP tag (2x)
backbone_originHomemade
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Reverse primer 2: CTTCCACCTCCAGAACCTCCACCCTTTTCGAATTGTGGATGGCTCC3 Reverse primer 3: GTGGATGGCTCCATGCGCTACCTCCACTTCCACCTCCAGAAC
CGAATTGTGGATGGCTCCAACCTCCATTAACGTTCTGAC
GTTC
Reverse primer 1:
growth strain :
Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.
origin :
37
pi :
alt_names
Protein G-related alpha 2M-binding protein
SPy_1357
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3See depositor comment
sequencing_primer_5ATGCCCATGGTTGATAGCCCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPy_1357, SPy1357
genegrab
id901432
genbank_ids
NP_269464.1
mutation
nameGRAB (V34-N181)
shRNA_sequence
size444
species
32644
Other
Streptococcus pyogenes serotype M1
tags
locationN terminal on insert
tagHis tag (6x)
locationC terminal on insert
tagStrep tag (2x)
resistance markers :
1612
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA