This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pIE-h 2M-H6
catalog :
128492
citations: 1
Reference
Marino Puertas L, Del Amo Maestro L, Taulés M, Gomis Ruth F, Goulas T. Recombinant production of human α2-macroglobulin variants and interaction studies with recombinant G-related α2-macroglobulin binding protein and latent transforming growth factor-β2. Sci Rep. 2019;9:9186 pubmed publisher
product information
Catalog Number :
128492
Product Name :
pIE-h 2M-H6
article :
doi10.1038/s41598-019-45712-z
id28203662
pubmed_id31235767
bacterial resistance :
Ampicillin
cloning :
After AgeI restriction site; introductionn of 6x-His and stop codon = ACCGGTcatcatcaccatcaccatTGAbackbone_originNovagenbackbone_size3843promotersequencing_primer_3sequencing_primer_5vector_types
Insect Expression

ATGTACAAGCTCACAGTCTTCCTGATGTTCATCGCTTTC
GTCATCATCGCTGAGGCCt
backbonepIEx
backbone_mutationIntroduction of T after the signal peptide to introduce StuI restriction site =
growth strain :
Expresses human 2M (from S24 to N1473) in insect cells. With C-t H6x tag.
origin :
37
pi :
alt_names
Alpha-2-macroglobulin
CPAMD5
FWP007
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5StuI
promoterIE1
sequencing_primer_3ATTACCGGTAGGATTTCCAAGATCTTTG
sequencing_primer_5GGCCTCAGTCTCTGGAAAACCGCAGTATATG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesA2MD, CPAMD5, FWP007, S863-7
geneA2M
id2
genbank_ids
CR749334
mutation
nameA2M
shRNA_sequence
size4350
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHis tag (6x)
resistance markers :
1612
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA