This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR_P4-P1R:PER36modp
catalog :
128431
citations: 1
Reference
McGee R, Dean G, Mansfield S, Haughn G. Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage. Plant Mol Biol. 2019;101:373-387 pubmed publisher
product information
Catalog Number :
128431
Product Name :
pDONR_P4-P1R:PER36modp
article :
doi10.1007/s11103-019-00909-8
id28203653
pubmed_id31422517
bacterial resistance :
Kanamycin
cloning :
backbonepDONR_P4-P1R
backbone_mutation
backbone_originInvitrogen
backbone_size5892
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway promoter entry clone
growth notes :
For use with Three-way Multi-site Gateway Cloning (Invitrogen).
growth strain :
Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).
growth temp :
For selection use 50 ug/ml Kanamycin
origin :
37
pi :
alt_names
PER36
PRX36
At3g50990
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterPEROXISADE36
sequencing_primer_35 -GAAGCATACCGAGCTAGAGC-3
sequencing_primer_55 -TGTAAAACGACGGCCAGT-3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G50990, PER36, peroxidase 36
geneAT3G50990
id824263
genbank_ids
BT010535
mutationPremature stop codons introduced into the Xero1 (cytosine to thymine mutation at 1,560 bp) and Xero2 (guanine to thymine mutation at 345 bp) genes in the PER36 promoter insert
namePEROXISADE36
shRNA_sequence
size2635
species
3702
Arabidopsis thaliana
tags
plasmid copy :
The PER36 promoter (2,635 bp) was originally cloned into the pDONR P4-P1R entry clone as described in Kunieda T, Shimada T, Kondo M, Nishimura M, Nishitani K, Hara-Nishimura I (2013) Spatiotemporal Secretion of PEROXIDASE36 Is Required for Seed Coat Mucilage Extrusion in Arabidopsis. Plant Cell 25:1355 1367. doi: 10.1105/tpc.113.110072
resistance markers :
4580
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA