This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONRG_P4-P1R:TBA2p
catalog :
128429
citations: 1
Reference
McGee R, Dean G, Mansfield S, Haughn G. Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage. Plant Mol Biol. 2019;101:373-387 pubmed publisher
product information
Catalog Number :
128429
Product Name :
pDONRG_P4-P1R:TBA2p
article :
doi10.1007/s11103-019-00909-8
id28203653
pubmed_id31422517
bacterial resistance :
Gentamicin
cloning :
backbonepDONRG_P4-P1R
backbone_mutation151 bp within the attR1 sequence there is a thymine to cysteine modification which does not impact the gateway reaction.
backbone_originInvitrogen modified by doi:10.1271/bbb.70678
backbone_size5892
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway promoter entry clone
growth notes :
For use with Three-way Multi-site Gateway Cloning (Invitrogen).
growth strain :
Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.
growth temp :
For selection use 7 ug/ml Gentamicin
origin :
37
pi :
alt_names
TBA2
AT1G62060.1
O04575 (Uniprot)
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterTESTA ABUNDANT 2
sequencing_primer_3M13R-pUC: 5 -CAGGAAACAGCTATGAC-3
sequencing_primer_5M13F: 5 -TGTAAAACGACGGCCAGT-3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G62060, F19K23.1, F19K23_1
geneAT1G62060
id842502
genbank_ids
AY125570.1
NCBI GeneID: 842502
mutation
nameTESTA ABUNDANT 2
shRNA_sequence
size1346
species
3702
Arabidopsis thaliana
tags
resistance markers :
4580
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA