This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
catalog :
128404
citations: 1
Reference
Stark J, Jaroentomeechai T, Moeller T, Hershewe J, Warfel K, Moricz B, et al. On-demand biomanufacturing of protective conjugate vaccines. Sci Adv. 2021;7: pubmed publisher
product information
Catalog Number :
128404
Product Name :
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
article :
doi10.1126/sciadv.abe9444
id28203640
pubmed_id33536221
bacterial resistance :
Spectinomycin and Streptomycin
cloning :
backbonepTrc99S
backbone_mutation
backbone_origin
backbone_size4535
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotertrc
sequencing_primer_3GATTTAATCTGTATCAGG
sequencing_primer_5GACAATTAATCATCCGGCTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPA1148
genetoxA
id877850
genbank_ids
mutation242DNNNS246 and 384DQNRT388 engineered glycosylation sites
nameExotoxin A
shRNA_sequence
size1906
species
32644
Other
P. aeruginosa
tags
locationC terminal on insert
tag6xHis tag
locationN terminal on backbone
tagDsbA signal sequence for periplasmic translocation
plasmid copy :
Exotoxin A with internal DNNNS and DQNRT glycosylation sites was originally described by Cuccui, et al. doi.org/10.1098/rsob.130002
resistance markers :
2546
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA