This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
catalog :
128387
citations: 1
Reference
Kilchrist K, Tierney J, Duvall C. Genetically encoded split-luciferase biosensors to measure endosome disruption rapidly in live cells. ACS Sens. 2020;: pubmed publisher
product information
Catalog Number :
128387
Product Name :
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
article :
doi10.1021/acssensors.0c00103
id28203639
pubmed_id32573202
bacterial resistance :
Ampicillin
cloning :
backbonepLV
backbone_mutation
backbone_originVectorBuilder
backbone_size7344
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Luciferase
Synthetic Biology
growth strain :
Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption
origin :
30
pi :
alt_names
Gal8
Galectin 8
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEFS
sequencing_primer_3
sequencing_primer_5None
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGal-8, PCTA-1, PCTA1, Po66-CBP
geneLGALS8
id3964
genbank_ids
NM_201545.2
mutation
nameNLuc398-3xGGGGS-LGALS8
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagNLuc398
alt_names
CALCOCO2
NDP52
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterIRES
sequencing_primer_3
sequencing_primer_5None
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNDP52
geneCALCOCO2
id10241
genbank_ids
NM_001261390.1
mutation
nameCALCOCO2-3xGGGGS-CLuc394
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagCLuc394
plasmid copy :
Outsourced synthesis
resistance markers :
4519
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA