pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394 | Addgene 128386 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

catalog :

128386

citations: 1

Reference
Kilchrist K, Tierney J, Duvall C. Genetically encoded split-luciferase biosensors to measure endosome disruption rapidly in live cells. ACS Sens. 2020;: pubmed publisher

product information

Catalog Number :

128386

Product Name :

pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

article :

doi	10.1021/acssensors.0c00103
id	28203639
pubmed_id	32573202

bacterial resistance :

Ampicillin

cloning :

backbone

pLV

backbone_mutation

EF1A promoter; EGFP:T2A:Bsd selection

backbone_origin

VectorBuilder

backbone_size

9096

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

Luciferase

Synthetic Biology

growth strain :

Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption

origin :

pi :

alt_names

Luc2

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	EF1A
sequencing_primer_3
sequencing_primer_5	none
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Gal-8, PCTA-1, PCTA1, Po66-CBP
gene	LGALS8
id	3964

genbank_ids

mutation

N terminal carbohydrate recognition domain fused to N terminal Luc fragment

name

NLuc-3xGGGGS-Gal8-NCRD

shRNA_sequence

size

1716

species

9606

Homo sapiens

100

Synthetic