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product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)
catalog :
128383
citations: 1
product information
Catalog Number :
128383
Product Name :
pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)
article :
| doi | 10.1038/s41594-018-0088-7 |
| id | 28203638 |
| pubmed_id | 29967542 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pGEX-6P1 | |
| backbone_mutation | ||
| backbone_origin | GE Healthcare | |
| backbone_size | 4960 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth strain :
expresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deleted
growth temp :
lower temperature to 16C for protein expression in BL21
origin :
37
pi :
L-UBC, UBCH7, UbcM4gene UBE2L3 id 7332 genbank_ids mutation mutation C86K in UbcH7 gene name UbcH7 shRNA_sequence size 1767 species
tags
promotorsequencing_pr
imer_3 gccaccgccggatccctggaagta
caggttttcaccgccGTCCACAGGTCGCTTTTCCCCd> sequencing_primer_5
TCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGg
atccATGGCGGCCAGCAGGAGGCTG
site_3_destroyed
site_5_destroyed
/tr>entrez_ge
ne
|
| ||||
|
promotor
imer_3
caggttttcaccgccGTCCACAGGTCGCTTTTCCCCd>
atccATGGCGGCCAGCAGGAGGCTG
/tr>
ne
|