This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
catalog :
128278
citations: 1
product information
Catalog Number :
128278
Product Name :
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
article :
| doi | 10.1016/j.celrep.2019.02.092 |
| id | 28203623 |
| pubmed_id | 30917306 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pet45b(+) | |
| backbone_mutation | ||
| backbone_origin | Novagen | |
| backbone_size | 5260 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth strain :
Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture
growth temp :
Grow with shaking at 225 rpm in Luria broth or 2X YT broth. For optimizing expression of the protein of interest, use appropriate E. coli protein expression strain such as BL21 (DE3) cells in 2X YT broth. Plasmid will be maintained at lower copy in these strains than in chemically-competent cloning strains such as E. coli Top10(TM) or DH10B(TM).
origin :
37
pi :
|
resistance markers :
| 4517 |
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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