pCMS-dsRed2-C1824U LMNA-GFP minigene | Addgene 128231 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMS-dsRed2-C1824U LMNA-GFP minigene

catalog :

128231

citations: 1

Reference
Shilo A, Tosto F, Rausch J, Le Grice S, Misteli T. Interplay of primary sequence, position and secondary RNA structure determines alternative splicing of LMNA in a pre-mature aging syndrome. Nucleic Acids Res. 2019;47:5922-5935 pubmed publisher

product information

Catalog Number :

128231

Product Name :

pCMS-dsRed2-C1824U LMNA-GFP minigene

article :

doi	10.1093/nar/gkz259
id	28203529
pubmed_id	31006814

bacterial resistance :

Ampicillin

cloning :

backbone

pCMS-EGFP

backbone_mutation

backbone_origin

Clontech

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

lamin A splicing reporter with short-lived GFP downstream LMNA exon 12 (CMV-driven) + SV40-driven dsRed2 to identified transfected cells. C>T mutation in exon 11. The mutation causes aberrant splicing event, creating a mutant protein, delta50 lamin A.

growth strain :

C1824U mutation Lamin A splicing reporter

origin :

pi :

alt_names

LMNA

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	EGFP-N
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
gene	LMNA
id	4000

genbank_ids

mutation

C1824U

name

lamin A

shRNA_sequence

size

850

species

9606

Homo sapiens