This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMS-dsRed2-WT LMNA-GFP minigene
catalog :
128230
citations: 1
Reference
Shilo A, Tosto F, Rausch J, Le Grice S, Misteli T. Interplay of primary sequence, position and secondary RNA structure determines alternative splicing of LMNA in a pre-mature aging syndrome. Nucleic Acids Res. 2019;47:5922-5935 pubmed publisher
product information
Catalog Number :
128230
Product Name :
pCMS-dsRed2-WT LMNA-GFP minigene
article :
doi10.1093/nar/gkz259
id28203529
pubmed_id31006814
bacterial resistance :
Ampicillin
cloning :
backbonepCMS-EGFP
backbone_mutation
backbone_originClontech
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
WT lamin A splicing reporter with short-lived GFP downstream LMNA exon 12 (CMV-driven) + SV40-driven dsRed2 to identified transfected cells.
growth strain :
Lamin A splicing reporter
origin :
37
pi :
alt_names
LMNA
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
geneLMNA
id4000
genbank_ids
mutation
namelamin A
shRNA_sequence
size850
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagGFP
location
tagSV40-driven dsRed2 to identify transfected cells.
plasmid copy :
Paula Scaffidi
resistance markers :
343
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA