This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PX458-sgEIF4EBP1
catalog :
128097
citations: 1
Reference
Walton Z, Patel C, Brooks R, Yu Y, Ibrahim Hashim A, Riddle M, et al. Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR. Cell. 2018;174:72-87.e32 pubmed publisher
product information
Catalog Number :
128097
Product Name :
PX458-sgEIF4EBP1
article :
doi10.1016/j.cell.2018.05.009
id28203585
pubmed_id29861175
bacterial resistance :
Ampicillin
cloning :
backbonePX458 (pSpCas9(BB)-2A-GFP)
backbone_mutation
backbone_originFeng Zhang
backbone_size9271
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP
origin :
37
pi :
alt_names
Eukaryotic translation initiation factor 4E-binding protein 1
4E-BP1
4EBP1
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterhU6
sequencing_primer_3
sequencing_primer_5U6-Fwd primer (GAGGGCCTATTTCCCATGATTCC)
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases4E-BP1, 4EBP1, BP-1, PHAS-I
geneEIF4EBP1
id1978
genbank_ids
NM_004095
mutation
nameCRISPR sgRNA against human 4EBP1
shRNA_sequenceTGAAGAGTCACAGTTTGAGA
size
species
9606
Homo sapiens
tags
plasmid copy :
GeCKO sgRNA library (Shalem et al., 2014) and PX458 (pSpCas9(BB)-2A-GFP, Feng Zhang, Addgene plasmid #48138)
resistance markers :
4516
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA