pGL4.22-VEGF-HRE::dLUC | Addgene 128096 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGL4.22-VEGF-HRE::dLUC

catalog :

128096

citations: 2

Reference
Yuan C, Liu J, Liu L, Jia H, Gao Q, Wang X, et al. TRIM7 suppresses cell invasion and migration through inhibiting HIF-1α accumulation in clear cell renal cell carcinoma. Cell Biol Int. 2021;: pubmed publisher
Walton Z, Patel C, Brooks R, Yu Y, Ibrahim Hashim A, Riddle M, et al. Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR. Cell. 2018;174:72-87.e32 pubmed publisher

product information

Catalog Number :

128096

Product Name :

pGL4.22-VEGF-HRE::dLUC

article :

doi	10.1016/j.cell.2018.05.009
id	28203585
pubmed_id	29861175

bacterial resistance :

Ampicillin

cloning :

backbone

pGL4.22

backbone_mutation

insertion of the VEGF HRE (x5) into promoter region

backbone_origin

Promega

backbone_size

5570

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth strain :

Hypoxia response element real-time luciferase reporter

growth temp :

The depositing lab used Top10 in their studies.

origin :

pi :

alt_names

HRE (x5), HIF response element (x5)

vascular endothelial growth factor hypoxia response element (x3)

VEGF, VEGFA

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	xhoI
promoter	inserted VEGF hypoxia response elements (HRE) (x5)
sequencing_primer_3
sequencing_primer_5	RVprimer3: CTAGCAAAATAGGCTGTCCC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	L-VEGF, MVCD1, VEGF, VPF
gene	VEGFA
id	7422

genbank_ids

NM_001025366

mutation

name

Hypoxia response element (x5) from VEGF promoter

shRNA_sequence

size

208

species

9606

Homo sapiens