This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL4.22-PGK1-HRE::dLUC
catalog :
128095
citations: 1
Reference
Walton Z, Patel C, Brooks R, Yu Y, Ibrahim Hashim A, Riddle M, et al. Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR. Cell. 2018;174:72-87.e32 pubmed publisher
product information
Catalog Number :
128095
Product Name :
pGL4.22-PGK1-HRE::dLUC
article :
doi10.1016/j.cell.2018.05.009
id28203585
pubmed_id29861175
bacterial resistance :
Ampicillin
cloning :
backbonepGL4.22
backbone_mutationinsertion of the PGK1 HRE (x3) into promoter region
backbone_originPromega
backbone_size5566
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
Hypoxia response element real-time luciferase reporter
growth temp :
The depositing lab used Top10 in their studies.
origin :
37
pi :
alt_names
HRE (x3)
HIF response element (x3)
phosphoglycerate kinase 1 hypoxia response element (x3)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5KpnI
promoterinserted PGK1 hypoxia response elements (HRE) (x3)
sequencing_primer_3
sequencing_primer_5RVprimer3: CTAGCAAAATAGGCTGTCCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-68p, MIG10, PGKA
genePGK1
id5230
genbank_ids
NM_000291
mutation
nameHypoxia response element (x3) from PGK1 promoter
shRNA_sequence
size79
species
9606
Homo sapiens
tags
locationC terminal on insert
tagdrives expression of destabilized luciferase (2CP) for real-time HRE activity assessment
plasmid copy :
HRE-pGL2-TK (Li et al., 2014; Celeste Simon Lab) and pGL4.22 (Promega)
resistance markers :
4516
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA