This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE
catalog :
127867
citations: 1
Reference
Candemir E, Kollert L, Weißflog L, Geis M, Müller A, Post A, et al. Interaction of NOS1AP with the NOS-I PDZ domain: Implications for schizophrenia-related alterations in dendritic morphology. Eur Neuropsychopharmacol. 2016;26:741-55 pubmed publisher
product information
Catalog Number :
127867
Product Name :
pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE
article :
doi10.1016/j.euroneuro.2016.01.008
id23166
pubmed_id26861996
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutation
backbone_origin
backbone_size5341
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
growth strain :
pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter
origin :
37
pi :
alt_names
CAPON
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NheI
promoterhSyn
sequencing_primer_3CATAGCGTAAAAGGAGCAACA
sequencing_primer_5CCCCGTAATGCAGAAGAAGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliases6330408P19Rik, Capon
geneNos1ap
id70729
genbank_ids
NM_001109985
NM_001164757
mutationconstructed by blunt end fusion of the 5 51 nucleotides (starting from the ATG) of the human NOS1AP (short isoform) ORF, to the 3 590 nucleotides of the mouse NOS1AP
nameNos1ap
shRNA_sequence
size641
species
9606
Homo sapiens
10090
Mus musculus
tags
locationN terminal on backbone
tagmCherry
locationN terminal on backbone
tag3xFLAG
plasmid copy :
The backbone was obtained from pAAV-hSyn-hChR2(H134R)-mCherry, a gift from Karl Deisseroth (Addgene plasmid # 26976). mCherry was amplified by PCR from pAAV-hSyn-hChR2(H134R)-mCherry, a gift from Karl Deisseroth (Addgene plasmid # 26976).
resistance markers :
4269
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA