pDSM-de-Ppgk1-PYC-Ttpi1 | Addgene 127735 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDSM-de-Ppgk1-PYC-Ttpi1

catalog :

127735

citations: 1

Reference
Young E, Zhao Z, Gielesen B, Wu L, Benjamin Gordon D, Roubos J, et al. Iterative algorithm-guided design of massive strain libraries, applied to itaconic acid production in yeast. Metab Eng. 2018;48:33-43 pubmed publisher

product information

Catalog Number :

127735

Product Name :

pDSM-de-Ppgk1-PYC-Ttpi1

article :

doi	10.1016/j.ymben.2018.05.002
id	28197680
pubmed_id	29753070

bacterial resistance :

Kanamycin

cloning :

backbone

pDSM-de

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

AAAGCAAAGGAAGGAGAGAAC

sequencing_primer_5

AAGCGACTTCCAATCGCTTTGC

vector_types

Yeast Expression

Synthetic Biology

growth notes :

PYC transcription unit with pathway position 5 homology arms, compatible with 5' position 4 and 3' position 6 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.

growth strain :

Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

origin :

pi :

alt_names

PYC

cloning

clone_method	Restriction Enzyme
cloning_site_3	BsaI
cloning_site_5	BsaI
promoter	Ppgk1
sequencing_primer_3	AGGTACAACAAGCACGACCG
sequencing_primer_5	AACGTTGTCCAGGTTTGTATCC
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	YGL062W
gene	PYC1
id	852818

genbank_ids

MH366513

mutation

name

pyruvate carboxylase

shRNA_sequence

size

3559

species

4932

Saccharomyces cerevisiae

100

Synthetic